This document specifies requirements and test methods for the dried barberry fruit of the Berberis vulgaris L. tree. It is applicable to dried red barberries only.

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This document specifies requirements and methods of sampling and testing for ground cassava leaves, which are obtained from the processing of fresh cassava leaves (Manihot esculenta Crantz or Manihot glaziovii) intended for human consumption.

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This document specifies the categories of pao cai (salted fermented vegetables) and its requirements, including sensory, physical and chemical, safety, labelling, transport and storage. It also describes the corresponding test methods. This document does not apply to kimchi.

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This document specifies the conditions required for good keeping of the following groups of citrus fruits during their storage with or without refrigeration, in stores or in various transport equipment (such as containers, railway cars, trucks or ships):
— oranges: Citrus sinensis (Linnaeus) Osbeck;
— mandarins: Citrus reticulata Blanco;
— lemons: Citrus limon (Linnaeus) N. L. Burman;
— grapefruits: Citrus paradisi Macfadyen;
— limes:  
— Citrus aurantifolia (Christmann) Swingle;
— Citrus latifolia Tanaka.  
Detailed information concerning cultivars in these different groups is given in Annexes A and B.

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This document specifies requirements and test methods for fresh artichokes, including their hearts and bottoms, of the following groups: — cynara cardunculus Scolymus Group; — cynara cardunculus Cardoon Group, syn. C. cardunculus var. altilis DC. It does not apply to processed artichokes.

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This document specifies requirements and test methods for fresh asparagus shoots of commercial varieties of asparagus grown from Asparagus officinalis L., of the Liliaceae family, offered to consumers after preparation and packaging. This document is applicable to all asparagus except green and violet/green asparagus with a diameter less than 3 mm and white and violet asparagus with a diameter less than 8 mm, packed in uniform bundles or unit packages. This document does not apply to processed asparagus.

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This document specifies requirements and test methods for the fresh cornelian fruit of the tree Cornus mas L. It does not apply to processed cornelian cherries.

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This document specifies requirements and test methods for priests (caper). It includes requirements for caper buds.

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This document specifies the conditions required for good keeping of the following groups of citrus fruits during their storage with or without refrigeration, in stores or in various transport equipment (such as containers, railway cars, trucks or ships): — oranges: Citrus sinensis (Linnaeus) Osbeck; — mandarins: Citrus reticulata Blanco; — lemons: Citrus limon (Linnaeus) N. L. Burman; — grapefruits: Citrus paradisi Macfadyen; — limes: — Citrus aurantifolia (Christmann) Swingle; — Citrus latifolia Tanaka. Detailed information concerning cultivars in these different groups is given in Annexes A and B.

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This European Standard specifies a high-performance liquid chromatographic (HPLC) and an ion chromatographic (IC) method for determination of the nitrate level in vegetables and vegetable products. This method is applicable for samples with a content of 25 mg/kg or greater.
It has been validated on naturally contaminated and spiked samples as beetroot juice with nitrate mass fractions of 194 mg/kg and 691 mg/kg, pureed carrots with nitrate mass fractions of 26 mg/kg and 222 mg/kg and with iceberg lettuce with nitrate mass fractions of 623 mg/kg and 3 542 mg/kg.

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This European Standard specifies a high-performance liquid chromatographic (HPLC) and an ion chromatographic (IC) method for determination of the nitrate level in vegetables and vegetable products. This method is applicable for samples with a content of 25 mg/kg or greater.
It has been validated on naturally contaminated and spiked samples as beetroot juice with nitrate mass fractions of 194 mg/kg and 691 mg/kg, pureed carrots with nitrate mass fractions of 26 mg/kg and 222 mg/kg and with iceberg lettuce with nitrate mass fractions of 623 mg/kg and 3 542 mg/kg.

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This Technical Specification specifies a method for the determination of acrylamide in cereal-based products, potato-based products and coffee by gas-chromatography mass spectrometry (GC-MS).
The method has been single-laboratory validated via the analysis of spiked samples (French fries (uncooked), bread, water biscuit, infant cereal, biscuit, green coffee, roast coffee and instant coffee), ranging from 30 μg/kg to 1 500 μg/kg acrylamide.
The results from the single laboratory validation were obtained by a laboratory with significant experience in acrylamide analysis. In addition, this method has also been studied by inter laboratory trial via the analysis of samples containing incurred acrylamide, ranging from approximately 200 μg/kg to 2 000 μg/kg. Critical points of the method are identified in 7.5 and Clause 8.

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This International Standard specifies a method that is applicable for the detection and enumeration of Cryptosporidium oocysts and Giardia cysts on or in food products that are described herein as fresh leafy green vegetables and berry fruits. With suitable controls, it may also be applicable for the examination of other fresh produce.
This method does not allow the determination of viability or infectivity of any Cryptosporidium oocysts and Giardia cysts which may be present. The microscopy descriptions are for Cryptosporidium oocysts and Giardia cysts of size ranges which include those species known to be pathogenic to humans.

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This European Standard specifies a method for the determination of acrylamide in bakery ware such as bread, toasted bread, crisp bread, butter cookies, and biscuits, as well as potato products such as potato chips, potato crisps, and potato pan cake and roasted coffee, by liquid chromatography in combination with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples, ranging from 14,3 µg/kg to 9 083 µg/kg. It was developed at the Swedish National Food Administration and validated in a study organized by the Directorate General Joint Research Centre (DG JRC), Swedish National Food Administration and the Nordic Committee on Food Analysis (NMKL), see [1] and [2].
The limit of quantification (LOQ) depends on the type of instrument used and on the actual performance of the instrument. The majority of the laboratories participating in the validation study were able to determine acrylamide in a butter cookie sample at a level of 14,3 µg/kg. Thus, the validation by interlaboratory study showed that LOQ can be expected to be in the range between below 15 µg/kg and 30 µg/kg.

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This European Standard gives guidance on some recommended techniques for the determination of pesticide residues in foods of plant origin and on confirmatory tests.
The identity of any observed pesticide residue is confirmed, particularly in those cases in which it would appear that the maximum residue limit has been exceeded.

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This European Standard gives general considerations for the determination of pesticide residues in foods of plant origin.
Each method specified in this European Standard is suitable for identifying and quantifying a definite range of those organohalogen, and/or organophosphorus and/or organonitrogen pesticides which occur as residues in foodstuffs of plant origin.
This European Standard contains the following methods that have been subjected to interlaboratory studies and/or are adopted throughout Europe:
-   method M: Extraction with acetone and liquid liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil® 1) [1], [2], [3];
-   method N: Extraction with acetone, liquid liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean-up with gel permeation and silica gel chromatography [4], [5];
-   method P: Extraction with ethyl acetate and, if necessary, clean-up with gel permeation chromatography [6].
The applicability of the three methods M, N and P for residue analysis of organohalogen, organophosphorus and organonitrogen pesticides, respectively, is given for each method.

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This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues.
Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used.
This European Standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and/or are adopted throughout Europe:
-   method M: Extraction with acetone and liquid-liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil®  ) [1], [2], [3];
-   method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean-up with gel permeation and silica gel chromatography [4], [5];
-   method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography [6].
This European Standard specifies the details of methods M, N and P for the extraction and the clean-up of food samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation chromatography.
A table providing the couples (matrix/pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible.

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This European Standard gives general considerations for the determination of pesticide residues in foods of plant origin. Each method specified in this European Standard is suitable for identifying and quantifying a definite range of those organohalogen, and/or organophosphorus and/or organonitrogen pesticides which occur as residues in foodstuffs of plant origin. This European Standard contains the following methods that have been subjected to interlaboratory studies and/or are adopted throughout Europe: - method M: Extraction with acetone and liquid-liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil® 1) [1], [2], [3]; - method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean-up with gel permeation and silica gel chromatography [4], [5]; - method P: Extraction with ethyl acetate and, if necessary, clean-up with gel permeation chromatography [6]. The applicability of the three methods M, N and P for residue analysis of organohalogen, organophosphorus and organonitrogen pesticides, respectively, is given for each method.

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This European Standard gives guidance on some recommended techniques for the determination of pesticide residues in foods of plant origin and on confirmatory tests. The identity of any observed pesticide residue is confirmed, particularly in those cases in which it would appear that the maximum residue limit has been exceeded.

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This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues. Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used. This European Standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and/or are adopted throughout Europe: - method M: Extraction with acetone and liquid-liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil® 1) [1], [2], [3]; - method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean-up with gel permeation and silica gel chromatography [4], [5]; - method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography [6]. This European Standard specifies the details of methods M, N and P for the extraction and the clean-up of food samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation chromatography. A table providing the couples (matrix/pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible.

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ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram.
The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.

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ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram.
The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.

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This International Standard specifies a method for the determination of the hydrochloric-acid-insoluble ash yielded by fruit and vegetable products.
The method serves for the determination of siliceous impurities, together with the silica endogenous to the plant.
NOTE A method for the determination of mineral impurities generally originating from the soil is specified in ISO 7621).

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This International Standard specifies a refractometric method for the determination of the soluble solids in fruit and vegetable products.
This method is particularly applicable to thick products, to products containing suspended matter, and to products rich in sugar. If the products contain other dissolved substances, the results will be only approximate; nevertheless, for convenience the result obtained by this method can be considered conventionally as the soluble solids content.
NOTE For the determination of the soluble solids in fruit juices (not containing suspended matter) and in concentrated juices (clarified), the pyknometric method specified in ISO 2172 is applicable.

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This International Standard specifies a method for determining the benzoic acid content of fruits, vegetables and derived products.
As chlorobenzoic acids are resistant to oxidation, the method cannot be applied in the presence of
p-chlorobenzoic acid, as the absorption spectrum of this acid is close to that of benzoic acid. Neither can it be used in the presence of cinnamic acid, which is transformed into benzoic acid by chromic acid oxidation.
NOTE The cinnamic acid determined as benzoic acid in this method exists generally only in the form of traces in vegetables, and therefore has no effect on the result obtained, except in the case of cinnamon bark, which contains higher quantities.

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This International Standard specifies a method for extracting the sorbic acid present in fruits, vegetables and derived products, and two techniques for determining the sorbic acid extracted.

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This international Standard specifies a method for the determination of the mineral impurities content (impurities generally originating from the soil) of fruit and vegetable products.

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This part of ISO 6561 specifies an atomic absorption spectrometric method for the determination of the cadmium content of fruits, vegetables and derived products.
NOTE The method of cadmium determination in fruit, vegetables and derived products is based on AOAC Official Methods of Analysis [1].

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This part of ISO 6561 specifies a graphite furnace atomic absorption spectrometric method for the determination of the cadmium content of fruits, vegetables and derived products.

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This European Standard specifies a method for the determination of patulin in fruit juices and fruit-based purée, such as baby food purée, using high performance liquid chromatography with ultra-violet detection (HPLC-UV). Using naturally contaminated and spiked samples this method has been validated for the determination of patulin in apple juice, at levels ranging from 3,0 μg/kg to 15,5 μg/kg, and in fruit-based baby food purée, at levels ranging from 3,4 μg/kg to 17,9 μg/kg. Baby food fruit purée used in this study contained a mixture of the following ingredients which are commercially available on the European market: blueberry; apple; banana; lemon; wheat biscuits; wheat syrup; whole milk; and vegetable oil. A detailed listing, including the fractions, of each product used in this study is given in. Further information on validation, see Clause 9 and Annex B.

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This European Standard specifies a method for the determination of patulin in fruit juices and fruit-based purée, such as baby food purée, using high performance liquid chromatography with ultra-violet detection (HPLC-UV). Using naturally contaminated and spiked samples this method has been validated for the determination of patulin in apple juice, at levels ranging from 3,0 μg/kg to 15,5 μg/kg, and in fruit-based baby food purée, at levels ranging from 3,4 μg/kg to 17,9 μg/kg. Baby food fruit purée (commercially available on the European market) used in this study contained the following ingredients: blueberry; apple; banana; lemon; wheat biscuits; wheat syrup; whole milk; and vegetable oil. A detailed listing, including the fractions, of each product used in this study is given in [1].
Further information on validation, see Clause 9 and Annex B.

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This European Standard specifies a method for the determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit and dried figs by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 1,1 µg/kg to 11 µg/kg.
For further information on the validation, see Clause 9 and Annex B.
WARNING - The use of this standard can involve hazardous materials, operations and equipment. This standard does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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This European Standard specifies a method for the determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit and dried figs by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 1,1 μg/kg to 11 μg/kg. For further information on the validation, see Clause 9 and Annex B.

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This European Standard is applicable to the determination of aflatoxins B1, B2, G1 and G2 in hazelnuts, figs, pistachios, peanuts and paprika powder. The limit of quantification of the method is 0,8 ng/g for each aflatoxin or better (value derived from in-house and collaborative study), depending on the equipment used.

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ISO 5519:2008 specifies a method for extracting the sorbic acid present in fruits, vegetables and derived products, and two techniques for determining the sorbic acid extracted.

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This European Standard is applicable to the determination of aflatoxins B1, B2, G1 and G2 in hazelnuts, figs, pistachios, peanuts and paprika powder. The limit of quantification of the method is 0,8 ng/g for each aflatoxin or better (value derived from in-house and collaborative study), depending on the equipment used.

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ISO 22855:2008 specifies a method using high-performance liquid chromatography for the determination of the concentration of benzoic and sorbic acids in fruit and vegetable juices.

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ISO 5518:2007 specifies a method for determining the benzoic acid content of fruits, vegetables and derived products. As chlorobenzoic acids are resistant to oxidation, the method cannot be applied in the presence of p-chlorobenzoic acid, as the absorption spectrum of this acid is close to that of benzoic acid. Neither can it be used in the presence of cinnamic acid, which is transformed into benzoic acid by chromic acid oxidation.

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This draft European Standard specifies a method using high performance liquid chromatography/mass spectrometry (LC-MS) for the determination of the growth regulators chlormequat and mepiquat in non fatty foods as chlormequat and mepiquat cation, respectively.
The method is applicable to all kinds of fruits, vegetables and cereal products. It has been collaboratively studied on mushrooms, pears, wheat flour and fruit puree, see [1].

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ISO 23391:2006 specifies requirements and test methods for dried rosehips obtained from the fruits of the rosehips shrub (Rosa canina L.) destined for human consumption.

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ISO 23392:2006 specifies a method for the determination of the alcohol-insoluble solids content of fresh or quick-frozen peas and maize, as well as whole kernel maize.

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ISO 23393:2006 specifies requirements and test methods for pomegranate fruit of the tree Punica granatum (L.) destined for human consumption.

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ISO 23394:2006 specifies requirements and test methods for dried oleaster obtained from the fruits of the tree Eleagnus angustifolia L. destined for human consumption.

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ISO 6561-1:2005 specifies a graphite furnace atomic absorption spectrometric method for the determination of the cadmium content of fruits, vegetables and derived products.

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ISO 6561-2:2005 specifies an atomic absorption spectrometric method for the determination of the cadmium content of fruits, vegetables and derived products.

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ISO 17239:2004 specifies a hydride generation atomic absorption spectrometric method for the determination of the arsenic content of fruits, vegetables and derived products.

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ISO 17240:2004 specifies an atomic absorption spectrometric method for the determination of the tin content of fruit and vegetable products in the concentration range 10 mg/kg to 500 mg/kg. It is a rapid method, especially suitable for routine determinations of tin in canned fruits and vegetables contaminated with tin which has migrated from the can. The method can be applied with the prescribed amount of sample to products with a maximum total dry matter content of 30 %. Products with higher contents of total solids can be analysed using smaller amounts of sample after corresponding dilution with deionized water.

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This European Standard specifies a method for the determination of patulin in apple juices and apple puree up to 128 ug/kg using high performance liquid chromatography (HPLC).

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This European Standard specifies a method for the determination of patulin in apple juices and apple puree up to 128 ug/kg using high performance liquid chromatography (HPLC).

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ISO 763:2003 specifies a method for the determination of the hydrochloric-acid-insoluble ash yielded by fruit and vegetable products. The method serves for the determination of siliceous impurities, together with the silica endogenous to the plant.

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