SIST EN ISO 10707:1998

SLOVENSKI STANDARD
SIST EN ISO 10707:1998
01-maj-1998
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SIST ISO 10707:1997
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Water quality - Evaluation in an aqueous medium of the "ultimate" aerobic
biodegradability of organic compounds - Method by analysis of biochemical oxygen
demand (closed bottle test) (ISO 10707:1994)
Wasserbeschaffenheit - Bestimmung der vollständigen aeroben biologischen
Abbaubarkeit organischer Stoffe in einem wäßrigen Medium - Verfahren mittels
Bestimmung des biochemischen Sauerstoffbedarfs (Geschlossener Flaschentest) (ISO
10707:1994)
Qualité de l'eau - Evaluation en milieu aqueux de la biodégradabilité aérobie ultime des
composés organiques - Méthode par anylyse de la demande biochimique en oxygene
(essai en fiole fermées) (ISO 10707:1994)
Ta slovenski standard je istoveten z: EN ISO 10707:1997
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN ISO 10707:1998 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 10707:1998

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SIST EN ISO 10707:1998

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SIST EN ISO 10707:1998

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SIST EN ISO 10707:1998

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SIST EN ISO 10707:1998

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SIST EN ISO 10707:1998
INTERNATIONAL
ISO
STANDARD
10707
First edition
1994-10-15
Water quality - Evaluation in an aqueous
medium of the “ultimate” aerobic
biodegradability of organic compounds -
Method by analysis of biochemical Oxygen
demand (closed bottle test)
Quake de I’eau - i%aluation en milieu aqueux de Ia biodegradabilitk
akobie wltime)) des composes organiques - Methode par analyse de
Ia demande biochimique en oxygkne (essai en fiole fermee)
Reference number
ISO 10707:1994(E)

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SIST EN ISO 10707:1998
EO ?0707:1994(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation of national Standards bodies (ISO member bodies). The work
of preparing International Standards is normally carried out through ISO
technical committees. Esch member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission
(1 EC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard ISO 10707 was prepared by Technical Committee
lSO/TC 147, Water quality, Subcommittee SC 5, Biological methods.
Annexes A, B and C of this International Standard are for information only.
0 ISO 1994
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronie or mechanicai, Inciudrng phorocopying and
mtcrofilm, without permrssion In writing from the publisher.
International Organization for Standardization
Case Postale 56 l CH-l 211 Geneve 20 l Switzerland
Printed rn Switzerland
ii

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SIST EN ISO 10707:1998
INTERNATIONAL STANDARD 0 ISO
ISO 10707:1994(E)
Water quality - Evaluation in an aqueous medium of
the “ultimate” aerobic biodegradability of organic
compounds - Method by analysis of biochemical
Oxygen demand (closed bottle test)
WARNING - SAFETY PRECAUTIONS - Activated sludge and sewage may contain potentially
pathogenic organisms. Therefore appropriate precautions should be taken when handling them.
Toxic test compounds and those whose properties are unknown should be handled with care.
are subject to revision, and Parties to agreements
1 Scope
based on this International Standard are encouraged
to investigate the possibility of applying the most re-
This International Standard specifies a method, by
cent editions of the Standards indicated below.
analysis of biochemical Oxygen demand, for the
Members of IEC and ISO maintain registers of cur-
evaluation in an aqueous medium of the “ultimate”
rently valid International Standards.
biodegradability of organic compounds at a given
concentration by aerobic microorganisms.
ISO 58137 983, Water quality - Determination of
dissolved Oxygen - lodome tric me thod.
The conditions described in this International Standard
do not necessarily always correspond to the optimal
ISO 5814:1990, Water quality - Determination of
conditions for allowing the maximum value of bio-
dissolved Oxygen
- Electrochemical probe method.
degradation to occur.
ISO 60603 989, Water quality - Determination of the
The method applies to all organic compounds which
are sufficiently water soluble to prepare a stock sol- Chemical Oxygen demand.
ution or poorly water soluble when using special dos-
ISO 9887:1992, Water quality - Evaluation of the
ing techniques.
aerobic biodegradability of organic compounds in an
Due to the low concentration of test compound at the
aqueous medium - Semi-continuous activated
beginning of the test, normally no special precautions
sludge method (SCAS).
for the toxicity of the test compound to the micro-
oroanisms of the inoculum is necessary; if required a ISO 9888: 1991, Wa ter quality - Evaluation of the
parallel inhibition test tan be performed. aerobic biodegradability of organic compounds in an
aqueous medium
- Static test (Zahn-WeIlens
me thod).
2 Normative references
ISO 10304-2: --J), Water quality - Determination of
dissolved anions by liquid chromatography of ions -
The following Standards contain provisions which,
Part 2: Determination o f bromide, chloride, nitra te,
through reference in this text, constitute provisions
nitrite, orthophosphate and sulfite in vvaste vvater.
sf this International Standard. At the time of publi-
cation, the editions indicated were valid. All Standards
1) To be published.

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SIST EN ISO 10707:1998
63 ISO
ISO 10707:1994(E)
ISO 10634:-IJ, Water quality - Guidance for the of dissolved Oxygen over a period of 28 d. The
amount of Oxygen taken up by the test Chemical
evaluation in an aqueous medium of the “ultjmate ”
soluble organic (BOD), corrected for uptake by the blank inoculum run
biodegradability of poorly
compounds. in parallel, is expressed as percentage of ThOD or
COD.
3 Definitions
5 Test environment
For the purposes of this International Standard, the
Incubation shall take place in the dark in an enclosure
following definitions apply.
which is maintained at a constant temperature (within
+ 1 “C) between 20 “C and 25 “C.
-
3.1 ultimate biodegradation: The level of degra-
dation achieved when the test compound is totally
utilized by microorganisms resulting in the production
6 Reagents
of carbon dioxide, water, mineral salts and new
microbial cellular constituents (biomass). Use only reagents of recognized analytical grade.
3.2 biochemical Oxygen demand (BOD): The mass
6.1 Water
concentration of dissolved Oxygen consumed under
specified conditions by the biological Oxidation of or-
Distilled or deionized water free from inhibitory con-
ganic and/or inorganic matter in water and is ex-
centrations of toxic substances containing less than
pressed in this case as milligrams of Oxygen uptake
10 % of the initial DOC content introduced by the
per milligram or gram of test compound.
compounds to be tested. For each series of tests, use
only one batch of water.
3.3 Chemical Oxygen demand (COD): The amount
of Oxygen consumed during Oxidation of a test com-
6.2 Test medium
pound with hot, acidic dichromate. lt provides a
measure of the amount of oxidizable matter present
and is expressed in this case as milligrams of Oxygen 6.2.1 Composition
consumed per milligram or gram of test compound.
6.2.1 . ‘l Solution a)
3.4 theoretical Oxygen demand (ThOD): The total
amount of Oxygen required to oxidize a Chemical
Anhydrous potassium dihydrogenphosphate
completely. lt is calculated from the molecular formula
KH,PO,) 65 g
and is expressed in this case as milligrams of Oxygen
Anhydrous dipotassium hydrogenphosphate
required per milligram or gram of test compound.
Zl,75 g
K2HP0,)
Disodium hydrogenphosphate dihydrate
3.5 pre-exposure (or pre-adaptation): The pre-
33,4 g
(Na,HPO,.ZH,O)
incubation of an inoculum in the presence of the test
Ammonium chloride (NH,CI)
Q5 g
compound, with the aim of enhancing the ability of
the inoculum to degrade the test compound. If the
Dissolve the ingredients in water (6.1) and make up
aim is achieved, the inoculum is said to be adapted.
to 1 000 ml.
3.6 pre-conditioning (or pre-acclimatization): The
NOTE ‘1 The correct composition of the medium tan be
pre-incubation of an inoculum under the conditions of
checked by the measurement of the pH-value, which should
the test in the absence of the test compound, to im- be 7.4.
prove the Performance of the test.
6.2.1.2 Solution b)
Dissolve 22,5 g of magnesium sulfate heptahydrate
4 Principle
(MgS0,.7H,O) in water (6.1) and dilute to 1 000 ml.
A Solution of the organic test compound in a mineral
medium as the sole Source of carbon and energy is
6.2A.3 Solution c)
inoculated with a relatively small number of micro-
Drssolve 27,5 g of anhydrous Calcium chlonde (CaCl,
organisms from a mixed population and kept in com-
pletely full, closed bottles in the dark at a constant or 36,4 g of calcrum chloride dihydrate iCaCl,.2H,O
temperature. Biodegradation is followed by analysis in water (6.11 and dilute to 1 000 ml.
2

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SIST EN ISO 10707:1998
0 ISO ISO 10707:1994(E)
bottles (7.3) containing a known volume of test me-
6.2.1.4 Solution d)
dium (6.2.2) so that the final concentration of the
Dissolve 0,25 g of iron(lll) chloride hexahydrate Chemical is normally 2 mg/l. This concentration is in
(FeCI,.GH,O) in water (6.1) and dilute to 1 000 ml. general suitable to ensure that the concentration of
Oxygen does not fall below 0,5 mg/1 during the test
In Order to avoid having to prepare this Solution im-
and the inoculum activity is not limited. For poorly
mediately before use, add one drop of concentrated
biodegradable compounds and those with a low
hydrochloric acid (HCI) or 0,4 g of ethylenediamine-
ThOD, up to IO mg/1 may be used.
tetraacetic acid (EDTA) (disodium salt) per Iitre.
In some cases, for example if a poor or partial degra-
dation is expected, it would be advisable to run paral-
6.2.2 Preparation of the test medium
lel series of test Chemical at two different
For 1 litre of test medium, add 1 ml of each of the
concentrations, for example 2 mg/1 and 5 mg/l.
solutions a) to d) (6.2.1) to about 500 ml of water
Normally, calculate the ThOD on the basis of forma-
(6.1) and adjust the volume to 1 000 ml.
tion of ammonium salts but, if nitrification is expected
or known to occur, calculate the ThOD on the basis
Strongly aerate the test medium for at least 20 min.
of the formation of nitrate. However, if nitrification is
Carry out each series of tests with test medium de-
not complete but does occur, correct for the changes
rived from the same batch. Generally, the medium is
in concentration of nitrite and nitrate, determined by
ready for use after standing for 20 h at the test tem-
analysis (see annex C).
perature. Determine the concentration of dissolved
Oxygen for control purposes; the value should be
In the case of test compounds that are very poorly
about 9 mg/1 at 20 “C. Conduct all transfer and filling
soluble in water for which no stock Solution tan be
operations of the air-saturated medium bubble-free, prepared, add the test compound in the required
for example, by the use of Siphons. quantity directly to the BOD bottles (7.1). Use the
large bottles (7.3) only to add inoculated test medium
(6.2.2). Avoid loss of test medium and test compound
7 Apparatus
when stoppering the bottles. For alternatives or more
details see ISO 10634.
Usual laboratory equipment, and
8.2 Preparation of reference compound
with glass Stoppers, of c apacity
7.1 BOD bottles
The bottles tan be made airtight Solution
250 ml to 300 ml.
case, only grease which is free of
by greasing. In this
Prepare a stock Solution of a known biodegradable
hall be
organic carbon, for . example Silicon grease, s
organic substance, for example sodium acetate,
used.
sodium benzoate or aniline, in the test medium
(6.2.2). In the same way as with the test compound,
7.2 Water bath or incubator, for keeping bottles
add a sufficient amount of the stock Solution to the
at a constant test temperature with the exclusion of
.
large bottles (7.3) to give a test concentration of
Irght .
2 mg/l.
7.3 Large glass bottles, of capacity 2 litres to
8.3 Preparation of inhibition control
5 litres, for the preparation of media and for filling the
BOD bottles.
If the toxicity of the test compound is to be investi-
gated (for example in the case of a previous low bio-
meter, or equ ipmen t for
7.4 Oxygen electrode and
degradability value having been found), another series
iodo metric Oxygen determina tion.
of bottles is necessary. Prepare another large bottle
(7.3) containing aerated mineral medium plus test
7.5 pH-meter.
compound and reference compound at final concen-
trations that are the same as those in the other large
bottles. Add the mixture to the BOD bottles (7.1).
8 Procedure
8.4 Preparation of the inoculum
8.1 Preparation of test compound solution
In this test use an inoculum without sludge flocs. lt
Prepare a stock Solution of the test compound in wa-
should be derived from the secondary effluent 0% a
ter (6.1) or test medium (6.2.2) (e.g. i g/l). Add a suf-
treatment plant or laboratory-scale unit receiving pre-
ficient amount of the stock Solution to the large
3

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SIST EN ISO 10707:1998
ISO %0707: ‘P994(E)
- at least IO bottles containing reference compound
dominantly domestic sewage or from surface watet-.
Mixtures from these different sources may also be and inoculum (FC);
used.
- at least IO bottles containing only inoculum (blank)
.
Collect a fresh Sample and keep it aerobic during
(FM I
transport. If suspended solids are present, allow to
settle for 1 h or filter through a coarse filter Paper and
and if necessary
keep the Sample under aerobic conditions until it is
required. - at least 6 bottles conta
...