SIST EN 17605:2022

SLOVENSKI STANDARD
SIST EN 17605:2022
01-julij-2022
Alge in izdelki iz alg - Metode vzorčenja in analize - Obdelava vzorca
Algae and algae products - Methods of sampling and analysis - Sample treatment
Algen und Algenprodukte - Methoden zur Probeentnahme und Analyse -
Probebehandlung
Algues et produits à base d’algues - Méthodes d’échantillonnage et d’analyse -
Traitement des échantillons
Ta slovenski standard je istoveten z: EN 17605:2022
ICS:
13.020.55 Biološki izdelki Biobased products
SIST EN 17605:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 17605:2022

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SIST EN 17605:2022


EN 17605
EUROPEAN STANDARD

NORME EUROPÉENNE

May 2022
EUROPÄISCHE NORM
ICS 13.020.55
English Version

Algae and algae products - Methods of sampling and
analysis - Sample treatment
Algues et produits à base d'algues - Méthodes Algen und Algenprodukte - Methoden zur
d'échantillonnage et d'analyse - Traitement des Probeentnahme und Analyse - Probebehandlung
échantillons
This European Standard was approved by CEN on 24 October 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17605:2022 E
worldwide for CEN national Members.

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EN 17605:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 10
5 Apparatus . 10
5.1 General. 10
5.2 Sample storage . 10
5.3 Ash content . 10
6 Reagents and materials . 11
7 Procedures . 11
7.1 General. 11
7.2 Handling of samples . 13
7.2.1 Sampling . 13
7.2.2 Transport . 13
7.2.3 Registration . 14
7.2.4 Storage . 14
7.2.5 Clean-up procedure (for wet samples) . 14
7.2.6 Homogenization . 14
7.2.7 Division into test samples . 14
7.2.8 Microbiological analysis . 14
7.2.9 Drying procedures . 14
7.3 Sample preparation . 15
7.4 Analysis . 16
7.5 Analysis of stored samples . 16
8 Calculations . 16
9 Quality control . 17
10 Report . 18
Annex A (informative) Example of a sample registration form . 19
Bibliography . 20

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EN 17605:2022 (E)
European foreword
This document (EN 17605:2022) has been prepared by Technical Committee CEN/TC 454 “Algae and
algae products”, the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2022, and conflicting national standards shall
be withdrawn at the latest by November 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
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Introduction
This document has been prepared by the experts of CEN/TC 454 “Algae and algae products”.
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European standards or European standardization deliverables to support the implementation of
Article 3 of Directive 2009/28/EC for algae and algae products.
1
This request, presented as Mandate M/547 , also contributes to the Communication on “Innovating for
Sustainable Growth: A Bio economy for Europe”.
The former working group CEN Technical Board Working Group 218 “Algae”, was created in 2016 to
develop a work programme as part of this Mandate. The technical committee CEN/TC 454 “Algae and
algae products” was established to carry out the work programme that will prepare a series of standards.
The interest in algae and algae products has increased significantly in Europe as a valuable source
including but not limited to carbohydrates, proteins, lipids, and several pigments. These materials are
suitable for use in a wide range of applications from food and feed purposes to other sectors, such as
textile, cosmetics, biopolymers, biofuel and fertilizer/biostimulants. Standardization was identified as
having an important role in order to promote the use of algae and algae products.
The work of CEN/TC 454 should improve the reliability of the supply chain, thereby improving the
confidence of industry and consumers in algae and algae products and will promote and support
commercialisation of the European algae industry.
This document has been developed with the aim to enable laboratories to conduct a standardized
procedure for sample preparation to be used for analysing samples of algae and algae products.

1
Available at http://ec.europa.eu/growth/tools-databases/mandates/index.cfm?fuseaction=refSearch.search#
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1 Scope
This document specifies the sample preparation of dry and wet samples of algae and algae products. This
document enables laboratories analysing algae samples to report accurate dry weight percentages and
to obtain representative samples possible for further examination.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17399:2020, Algae and algae products - Terms and definitions
EN ISO 24333:2009, Cereals and cereal products — Sampling
EN ISO 5667-1:2006, Water quality — Sampling — Part 1: Guidance on the design of sampling programmes
and sampling techniques
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 17399:2020 and the following
apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
lot
quantity of material that is assumed to be of the same production process and represented by specified
sampling rules
[SOURCE: EN ISO 6498:2012, definition 2.1.1]
3.2
laboratory sample
sample as prepared (from the lot) for sending to the laboratory and intended for inspection or testing
[SOURCE: EN ISO 6498:2012, definition 2.1.2]
3.3
test sample
subsample or sample prepared from the laboratory sample and from which test portions will be taken
[SOURCE: EN ISO 6498:2012, definition 2.1.3]
3.4
test portion
quantity of material drawn from the test sample (or from the laboratory sample if both are the same)
[SOURCE: EN ISO 6498:2012, definition 2.1.4]
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3.5
reserve sample
material left over from the laboratory sample when divided or subsampled test samples have been taken
and on which no further particle size reduction is done
[SOURCE: EN ISO 6498:2012, definition 2.1.5, modified – the note is left out]
3.6
sample
laboratory sample, a test sample, a test portion or a reserve sample as described in (3.2), (3.3), (3.4), (3.5)
3.7
dry sample
sample with a moisture content ≤15 % of the total mass
3.8
wet sample
sample with a moisture content > 15 % of the total mass
3.9
homogeneity
degree to which a property or a constituent is uniformly distributed throughout a quantity of material
Note 1 to entry: Homogeneity may be considered to having been achieved in a practical sense when the sampling
error of the processed portion is negligible compared to the total error of the measurement system. Since
homogeneity depends on the size of the units under consideration, a mixture of two materials may be
inhomogeneous at the molecular or atomic level, but sufficiently homogeneous at the particulate level. However,
uniform visual appearance does not ensure compositional homogeneity.
[SOURCE: EN ISO 6498:2012, definition 2.4.1]
3.10
homogenization
procedure to assure the homogeneity of the sample, including coarse grinding, fine grinding, mixing,
agitating, blending and pulverization
3.11
partial drying
drying
drying procedure for wet samples, in which the sample is carefully dried to allow subsequent sample
preparation procedures to be applied
Note 1 to entry: Partial drying can be performed by oven drying or freeze drying and is presented in % moisture
content reduction.
3.12
coarse grinding
first grinding step of the whole sample when the laboratory sample contains large lumps or when its
particle size is above about 6 mm before mass reduction
Note 1 to entry: Coarse grinding is a special kind of particle size reduction that ensures homogeneity of the
laboratory sample for subsampling purposes.
[SOURCE: EN ISO 6498:2012, definition 2.4.3]
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3.13
fine grinding
grinding procedure for reducing the particle size to the requested size <500 µm, which is achieved by
chopping, crushing, cutting, macerating, milling (grinding), pressing to obtain a homogeneous test sample
for further analysis
3.14
subsampling (splitting)
dividing the pre-treated and homogenized laboratory sample into the test sample and the reserve sample
Note 1 to entry: After subsampling (splitting), all subsamples should have the same properties as the original
laboratory sample.
Note 2 to entry: Definition is similar to definition of mass reduction (EN ISO 6498:2012).
3.15
true value
value which would be obtained under ideal measuring conditions where no errors occur
3.16
moisture content
loss on drying
mass volatile components of the sample lost after drying under test conditions / initial mass
3.17
dry weight (d.w.)
mass corrected for 100 % dry matter (i.e. a moisture content of 0 %)
3.18
parameter
analyte or constituent or microorganism for which the sample is to be analysed by microscopic,
microbiological, biological or chemical procedures
[SOURCE: EN ISO 6498:2012, definition 2.2.1]
3.19
stable parameter
analyte or constituent or microorganism which does not degrade during sample preparation on common
handling or storage at room temperatures of 20 °C to 25 °C
[SOURCE: EN ISO 6498:2012, definition 2.2.1.1]
3.20
unstable parameter
analyte or constituent or microorganism which degrades during sample preparation on common
handling or storage at room temperatures of 20 °C to 25 °C because they are volatile, degradable, or
sensitive to temperature, light, enzymatic degradation or chemical oxidation
[SOURCE: EN ISO 6498:2012, definition 2.2.1.2]
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3.21
constant mass
constant mass is reached when, during the drying process the difference between two successive
weighings of the sample, first heated, then cooled to room temperature and with an interval of 1 h
between them, does not exceed 0,5 % (m/m) of the last determined mass or 2 mg, whichever is the
greater
[SOURCE: EN 12880:2000, definition 3.4]
NOTE The relations between the different definitions are given in Figure 1.
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Figure 1 — Illustration of relation between the different samples types and sample preparation
procedures
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4 Principle
Proper sample preparation is one of the crucial steps in obtaining the best possible result that most likely
corresponds to the “true value”. All sample treatment steps depend on the different properties of the
sample material and o
...