SIST EN ISO 3596:2001

SLOVENSKI STANDARD
SIST EN ISO 3596:2001
01-december-2001
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHQHXPLOMLYLKVQRYL0HWRGD
HNVWUDNFLMH]GLHWLOHWURP5HIHUHQþQDPHWRGD,62
Animal and vegetable fats and oils - Determination of unsaponifiable matter - Method
using diethyl ether extraction
Tierische und pflanzliche Fette und Öle - Bestimmung der unverseifbaren Bestandteile -
Verfahren mit Diethylether-Extraktion
Corps gras d'origines animale et végétale - Détermination de la teneur en matieres
insaponifiables - Méthode par extraction a l'oxyde diéthylique
Ta slovenski standard je istoveten z: EN ISO 3596:2001
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 3596:2001 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 3596:2001

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SIST EN ISO 3596:2001
EUROPEAN STANDARD
EN ISO 3596
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2001
ICS 67.200.10 Supersedes EN ISO 3596-1:2000
English version
Animal and vegetable fats and oils - Determination of
unsaponifiable matter - Method using diethyl ether extraction
Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung der
de la teneur en matières insaponifiables - Méthode par unverseifbaren Bestandteile - Verfahren mit Diethylether-
extraction à l'oxyde diéthylique Extraktion
This European Standard was approved by CEN on 25 June 2001.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 3596:2001 E
worldwide for CEN national Members.

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SIST EN ISO 3596:2001
EN ISO 3596:2001 (E)
CORRECTED 2001-12-05
Foreword
The text of the International Standard from Technical Committee ISO/TC 34 "Agricultural food
products" of the International Organization for Standardization (ISO) has been taken over as an
European Standard by Technical Committee CEN/TC 307 "Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis", the secretariat of which is
held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by February 2002, and conflicting national
standards shall be withdrawn at the latest by February 2002.
This European Standard replaces EN ISO 3596-1:2000.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium, Czech
Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg,
Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of the International Standard ISO 3596:2000 has been approved by CEN as a
European Standard without any modification.
NOTE: Normative references to International Standards are listed in annex ZA (normative).
2

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SIST EN ISO 3596:2001
EN ISO 3596:2001 (E)
Annex ZA (normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions of
-any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications, indicated
by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 661 1989 Animal and vegetable fats and oils - EN ISO 661 1995
Preparation of test sample
3

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SIST EN ISO 3596:2001

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SIST EN ISO 3596:2001
INTERNATIONAL ISO
STANDARD 3596
First edition
2000-07-01
Animal and vegetable fats and oils —
Determination of unsaponifiable matter —
Method using diethyl ether extraction
Corps gras d'origines animale et végétale — Détermination de la teneur en
matières insaponifiables — Méthode par extraction à l'oxyde diéthylique
Reference number
ISO 3596:2000(E)
©
ISO 2000

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SIST EN ISO 3596:2001
ISO 3596:2000(E)
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ii © ISO 2000 – All rights reserved

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SIST EN ISO 3596:2001
ISO 3596:2000(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 3596 was prepared by Technical Committee ISO/TC 34, Agricultural food products,
Subcommittee SC 11, Animal and vegetable fats and oils.
This first edition of ISO 3596 cancels and replaces ISO 3596-1:1988 and its Amendment 1:1997, of which it
constitutes a minor revision.
Annex A of this International Standard is for information only.
© ISO 2000 – All rights reserved iii

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SIST EN ISO 3596:2001

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SIST EN ISO 3596:2001
INTERNATIONAL STANDARD ISO 3596:2000(E)
Animal and vegetable fats and oils — Determination of
unsaponifiable matter — Method using diethyl ether extraction
1 Scope
This International Standard specifies a method using diethyl ether extraction for the determination of the
unsaponifiable matter content of animal and vegetable fats and oils.
This method is not applicable to waxes and, moreover, gives approximate results with certain fats of high
unsaponifiable matter content, for example with fats derived from marine animals.
A method given in ISO 18609 may be used when climatic conditions, or regulations, do not permit the use of diethyl
ether.
2 Normative reference
The following normative document contains provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent edition of the normative document indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 661, Animal and vegetable fats and oils — Preparation of test sample.
3 Term and definition
For the purposes of this International Standard, the following term and definition applies.
3.1
unsaponifiable matter
all the substances present in the product which, after saponification of the latter by potassium hydroxide and
extraction by a specified solvent, are not volatile under the specified operating conditions
4Principle
The fat or oil is saponified by boiling under reflux with an ethanolic potassium hydroxide solution. The
unsaponifiable matter is extracted from the soap solution by diethyl ether. The solvent is evaporated and the
residue is weighed after drying.
5 Reagents
Use only reagents of recognized analytical grade and distilled or deionized water or water o
...