SIST EN ISO 11063:2020

Oznaka standarda: SIST EN ISO 11063:2020
Koda projekta: 00444174
Organizacija: SIST
Naslov (angleški): Soil quality - Direct extraction of soil DNA (ISO 11063:2020)
Naslov (slovenski): Kakovost tal - Neposredna ekstrakcija DNK iz vzorcev tal (ISO 11063:2020)
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Ime Jezik Status Cena Dodaj v košarico
SIST EN ISO 11063:2020 angleški jezik Active SIST-E: 58.08 EUR
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Tehnični odbor: KAT - Karakterizacija tal, odpadkov in blata
ICS: 13.080.30
Status: Objavljen
Objavljen: 01-dec-2020
Refer. št. objave: Sporocila 2020-12
Referenčna oznaka: EN ISO 11063:2020
Področje projekta (angleško): The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals. The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.
Povezava na standarde
Povezava Ime Datum
Nadomešča SIST EN ISO 11063:2013 - Kakovost tal - Metoda neposredne ekstrakcije DNK iz vzorcev tal (ISO 11063:2012) 01-dec-2020


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