Oilseeds -- Determination of hexane extract (or light petroleum extract), called "oil content"

Graines oléagineuses -- Détermination de l'extrait à l'hexane (ou à l'éther de pétrole), dit "teneur en huile"

Oljnice - Določanje heksanskega (ali petroleterskega) ekstrakta, imenovanega "vsebnost olja"

General Information

Status
Withdrawn
Publication Date
31-May-1995
Withdrawal Date
31-Aug-2001
Current Stage
9900 - Withdrawal (Adopted Project)
Start Date
01-Sep-2001
Due Date
01-Sep-2001
Completion Date
01-Sep-2001

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IS0
INTERNATIONAL STANDARD
659
Second edition
1988-02- 15
INTERNATIONAL ORGANIZATION FOR STANDARDIZATION
ORGANISATION INTERNATIONALE DE NORMALISATION
ME>KAYHAPOflHAR OPrAHM3A!&iR il0 CTAHflAPTM3AuMM
- Determination of hexane extract (or light
Oilseeds
petroleum extract), called "oil content"
Graines oléagineuses - Détermination de I'extrait à I'hexane (ou à I'éther de pétrole),
dit ((teneur en huile))
Reference number
IS0 659 : 1988 (E)

---------------------- Page: 1 ----------------------
Foreword
IS0 (the International Organization for standardization) is a worldwide federation of
national standards bodies (IS0 member bodies). The work of preparing International
Standards is normally carried out through IS0 technical committees. Each member
body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, govern-
mental and non-governmental, in liaison with EO, also take part in the work. IS0
collaborates closely with the International Electrotechnical Commission (IEC) on all
matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are circulated to
the member bodies for approval before their acceptance as International Standards by
the IS0 Council. They are approved in accordance with IS0 procedures requiring at
least 75 % approval by the member bodies voting.
International Standard IS0 659 was prepared by Technical Committee ISO/TC 34,
Agricultural food products.
This second edition cancels and replaces the first edition (IS0 659 : 19791, of which it
constitutes a minor revision.
International Organization for Standardization. 1988 0
Printed in Switzerland

---------------------- Page: 2 ----------------------
INTERNATIONAL STANDARD IS0 659 : 1988 (E)
Oilseeds - Determination of hexane extract (or light
petroleum extract), called "oil content"
5 Reagents
1 Scope
5.1 Technical n-hexane or, failing this, light petroleum,
This International Standard specifies a reference method for
essentially composed of hydrocarbons with 6 carbon atoms, of
the determination of the hexane extract (or light petroleum
which less than 5 YO distils below 50 OC and more than 95 %
extract), called "oil content", of oilseeds used as industrial raw
distils between 50 and 70 OC and which has a bromine value
materials.
less than 1. For either solvent, the residue on complete
U'
evaporation shall not exceed 2 mg per 100 ml.
NOTE - If required, the following may be analysed separately :
-
the pure seeds and the impurities (see 11.2) :
5.2 Hydrochloric acid, concentrated, e20 = 1,19 g/ml
-
in the case of groundnuts. the pure seeds, the total fines, the
(only in the case of cottonseed with adherent linters -
non-oleaginous impurities and the oleaginous impurities.
see 8.4).
2 Normative references
6 Apparatus
The following standards contain provisions which, through
Usual laboratory apparatus and in particular :
reference in this text, constitute provisions of this International
Standard. At the time of publication, the editions indicated
6.1 Analytical balance.
were valid. All standards are subject to revision, and parties to
agreements based on this International Standard are encour-
6.2 Mechanical mill, easy to clean, appropriate to the
aged to investigate the possibility of applying the most recent
nature of the oilseeds and allowing the oilseeds to be ground
editions of the standards listed below. Members of IEC and IS0
without heating or appreciable change in moisture, volatile
maintain registers of currently valid International Standards.
matter or oil content.
IS0 542 : 1980, Oilseeds - Sampling.
6.3 Mechanical grater or, failing this, hand grater (only in
the case of copra - see 8.2).
IS0 664 : 1977, Oilseeds - Reduction of contract samples to
<- analysis samples.
6.4 Mechanical micro-grinder (see 11.11, capable of pro-
ducing a fineness of grinding of oilseeds of less than 160 pm,
IS0 665 : 1977, Oilseeds - Determination of moisture and
with the exception of the "shell", particles of which may reach
volatile matter content.
400 Hm.
6.5 Extraction thimble and cotton wool, free from matter
3 Definition
soluble in hexane or light petroleum.
For the purposes of this International Standard, the following
6.6 Suitable extraction apparatus, fitted with a flask of
definition applies.
capacity 200 to 250 ml.
hexane extract, called "oil content": The whole of the
NOTE - Several flasks are necessary (see 9.3).
substances extracted under the operating conditions specified
in this International Standard, and expressed as a percentage
6.7 Pumice stone, in small particles, previously dried in an
by mass of the product as received. On request, it may be
oven at 103 OC I 2 OC and cooled in a desiccator.
expressed relative to the dry matter.
6.8 Electric heating bath (sand-bath, water-bath, etc.) or
hot-plate.
4 Principle
6.9 Electrically heated oven, with thermostatic control,
Extraction of a test portion in a suitable apparatus, with
capable of being maintained in particular at 103 OC I 2 OC,
technical hexane or, failing this, light petroleum. Elimination of
permitting ventilation or reduced pressure to be obtained.
the solvent and weighing of the extract obtained.
1

---------------------- Page: 3 ----------------------
IS0 659 : 1988 (E)
6.10 Desiccator, containing an efficient desiccant. 8.4 Cottonseed with adherent linters
Weigh, to the nearest 1 mg in the tared metal dish (6.11.31,
6.11
In the case of cottonseed with adherent linters, the
about 60 g of the analysis sample as received. Place the dish
following are also required.
and seeds in the oven (6.11.11, previously heated to 130 OC,
and leave to dry for 2 h at 130 OC f 2 OC; then remove the
6.11.1 Electrically heated oven, capable of being main-
dish from the oven and allow to cool in air for about 30 min.
tained at 130 OC f 2 OC.
Transfer the dried seeds to the porous ceramic vessel (6.11.4),
the inside walls and the base of which have been previously
moistened with 1,5 ml of the hydrochloric acid (5.2) by means
6.11.2 Fumigation oven, with a thermostatic control,
of the pipette (6.11.61, taking care that the acid is completely
capable of heating a sample to 115 OC in 30 min.
absorbed without forming adherent drops. Cover the vessel
with the watch glass (6.11.5) and place it in the fumigation
6.11.3 Metal dish, flat-bottomed, of diameter 100 mm and
oven (6.1 1.2). Heat so as to reach 115 OC in 30 min; do not heat
height approximately 40 mm.
beyond this temperature and maintain it for another 30 min.
6.11.4 Porous vessel, made of ceramic material, cylindrical, Remove the vessel from the oven, allow to cool for 1 h in air,
of internal diameter 68 mm, external diameter 80 mm, height reweigh the treated seeds to the nearest 1 mg, then grind the
85 mm, and thickness of walls and base 6 mm. seeds in the mechanical mill (6.2) and proceed as specified
in 8.3.
6.11.5 Watch glass, of diameter 80 to 90 mm.
8.5 Small seeds (linseed, colza, etc.)
6.11.6 Pipette, 2 ml, graduated in 0,l ml divisions.
Carefully mix the analysis sample without previous mechanical
grinding.
7 Sampling
Sampling shall be carried out in accordance with IS0 542.
9 Procedure
8 Preparation of test sample
9.1 Test portion
8.1 Reduction of sample
9.1.1 The test portion shall be representative of the analysis
sample.
Take an analysis sample obtained in accordance with IS0 664.
If large non-oleaginous foreign bodies have been separated
1 mg, about 10 g of the test sam-
9.1.2 Weigh, to the nearest
before the reduction of the laboratory sample, make allowance
ple (see 8.2, 8.3, 8.4 or 8.5, as appropriate).
for this in the calculation (see 10.1.4). According to-the re-
quirements of the contract, use an analysis sample as received
NOTE - In the case of groundnuts. the test portion may comprise the
or after separation of the impurities.
separated fractions of pure seeds. non-oleaginous and oleaginous im-
purities, and the total fines in quantities proportional to those of the dif-
-
8.2 Copra
ferent constituents in the analysis sample itself
Grate the product by hand or, preferably, using a mechanical
9.1.3 In the case of copra and medium-sized seeds, including
grater (6.3) which allows the whole sample to be treated. When
test portion to
cottonseed with adherent linters, transfer the
grating by hand (a process which does not allow all the analysis
the thimble (6.5) and plug the latter with a wad of cotton wool
sample to be grated), endeavour to obtain a test sample which
(6.5).
is as representative as possible and, to this end, take account of
the size and colour of different fragments.
9.1.4 In the case of small seeds, grind the test portion in the
micro-grinder (6.4) or in the mill (6.21, taking care not to leave
The length of the particles shall be close to 2 mm but shall not
be greater than 5 mm. Mix the particles carefully and carry out any seeds intact. Transfer the ground seeds, without loss, to
the thimble (6.5), using a spatula. Wipe the bowl of the micro-
the determination without delay.
grinder or mill and the spatula with a wad of cotton wool (6.5)
soaked with solvent (5.1), and plug the thimble with this wad.
8.3 Seeds of medium-size (sunflower, groundnut,
soya, etc.)
9.2 Predrying
Except in the case of cottonseed with adherent linters, grind
the analysis sample in, the mechanical mill (6.2), which has If the test portion is very moist [moisture and volatile matter
content above 10 % (rn/rn)l, leave the filled thimble for some
previously been well cleaned, until the major dimension of the
particles obtained is not greater than 2 mm. Reject the first par- time in an oven, maintained at a temperature not higher than
80 OC, to reduce the moisture and volatile matter content to
ticles (about one-twentieth of the sample), co
...

IS0
NORME INTERNATIONALE
659
Deuxième édition
1988-02-15
INTERNATIONAL ORGANIZATION FOR STANDARDIZATION
ORGANISATION INTERNATIONALE DE NORMALISATION
MEXAYHAPOAHAR OPiAHM3AuMR Il0 CTAHAAPTMSALWM
Graines oléagineuses - Détermination de l'extrait à
I'hexane (ou à l'éther de pétrole), dit «teneur en huile»
Oilseeds - Determination of hexane extract (or light petroleum extract), called 'oil content"
Numéro de référence
IS0 659 : 1988 (F)

---------------------- Page: 1 ----------------------
Avant-propos
L‘ISO (Organisation internationale de normalisation) est une fédération mondiale
d‘organismes nationaux de normalisation (comités membres de I’ISO). L’élaboration
des Normes internationales est en général confiée aux comités techniques de I’ISO.
Chaque comité membre intéressé par une étude a le droit de faire partie du comité
technique créé à cet effet. Les organisations internationales, gouvernementales et non
gouvernementales, en liaison avec 1’1S0 participent également aux travaux. L‘ISO col-
labore étroitement avec la Commission électrotechnique internationale (CE11 en ce qui
concerne la normalisation électrotechnique.
Les projets de Normes internationales adoptés par les comités techniques sont soumis
aux comités membres pour approbation, avant leur acceptation comme Normes inter-
nationales par le Conseil de I‘ISO. Les Normes internationales sont approuvées confor-
mément aux procédures de I’ISO qui requièrent l’approbation de 75 % au moins des
comités membres votants.
La Norme internationale IS0 659 a été élaborée par le comité technique ISO/TC 34,
Produits agricoles alimentaires.
Cette deuxième édition annule et remplace la première édition (IS0 659 : 1979), dont
elle constitue une révision mineure.
O Organisation internationale de normalisation, 1988 O
ImDrirné en Suisse

---------------------- Page: 2 ----------------------
~~
NORME INTERNATIONALE IS0 659 : 1988 (FI
Graines oléagineuses - Détermination de l'extrait à
I'hexane (ou à l'éther de pétrole), dit ((teneur en huile»
5 Réactifs
1 Domaine d'application
5.1 n-Hexane technique, ou, à défaut, éther de pétrole,
La présente Norme internationale spécifie une méthode de réfé-
essentiellement constitué d'hydrocarbures à 6 atomes de car-
rence pour la détermination de l'extrait à I'hexane (ou à l'éther
bone dont moins de 5 % distillent au-dessous de 50 OC et plus
de pétrole), dit ((teneur en huile)), des graines oléagineuses uti-
de 95 YO distillent entre 50 et 70 OC, et ayant un indice de
lisées comme matière première industrielle.
brome inférieur à 1. Le résidu à l'évaporation complète doit
L
être, pour les deux solvants, inférieur à 2 mg pour 100 ml.
NOTE - Sur demande, on peut analyser séparément
- les graines pures et les impuretés (voir 11.21 ;
5.2 Acide chlorhydrique, concentré, = 1,19 g/ml (seule-
- les graines pures, les poussières totales, les impuretés non
ment dans le cas des graines de coton à linters adhérents -
oléagineuses et oléagineuses, dans le cas de l'arachide.
voir 8.4).
2 Références normatives
6 Appareillage
Les normes suivantes contiennent des dispositions qui, par
suite de la référence qui en est faite, constituent des disposi-
Matériel courant de laboratoire, et notamment:
tions valables pour la présente Norme internationale. Au
moment de la publication de cette norme, les éditions indiquées
étaient en vigueur. Toute norme est sujette à révision et les par- 6.1 Balance analytique.
ties prenantes des accords fondés sur cette Norme internatio-
nale sont invitées à rechercher la possibilité d'appliquer les édi-
6.2 Broyeur mécanique, facile à nettoyer, approprié à la
tions les plus récentes des normes indiquées ci-après. Les
nature des graines oléagineuses et permettant le broyage de
membres de la CE1 et de I'ISO possèdent le registre des Normes
celles-ci sans échauffement et sans modification sensible de
internationales en vigueur à un moment donné.
leur teneur en eau, en matières volatiles et en huile.
IS0 542 : 1980, Graines oléagineuses - Échantillonnage.
6.3 Râpe mécanique ou, à défaut, manuelle (seulement
L
IS0 ô64 : 1977, Graines oléagineuses - Réduction des échan-
dans le cas du coprah - voir 8.2).
tillons pour laboratoire en échantillons pour analyse.
IS0 665 : 1977, Graines oléagineuses - Détermination de la
6.4 Microbroyeur mécanique, (voir 11.1 1, capable de pro-
teneur en eau et matières volatiles.
duire une finesse de mouture des graines oléagineuses infé-
rieure à 160 pm, à l'exception de II« enveloppe )) dont les parti-
cules peuvent atteindre 400 vm.
3 Définition
Pour les besoins de la présente Norme internationale, la défini- 6.5 Cartouche à extraction et ouate, exemptes de matiè-
tion suivante s'applique.
res solubles dans I'hexane ou dans l'éther de pétrole.
extrait à I'hexane, dit ((teneur en huile)) : Totalité des subs-
6.6 Appareil à extraction approprié, muni d'un ballon de
tances extraites dans les conditions opératoires spécifiées dans
200 à 250 ml de capacité.
la présente Norme internationale et exprimée en pourcentage
en masse rapporté au produit tel quel. Sur demande, il peut
NOTE - II est nécessaire d'avoir plusieurs ballons (voir 9.31
être exprimé par rapport à la matière sèche.
Pierre ponce, en petits grains, séchée dans une étuve à
6.7
4 Principe
103 OC + 2 OC et refroidie dans un dessiccateur.
Extraction d'une prise d'essai dans un appareil approprié, avec
6.8 Bain à chauffage électrique (bain de sable, bain d'eau,
de I'hexane technique ou, à défaut, de l'éther de pétrole. Élimi-
nation du solvant d'extraction et pesée de l'extrait obtenu. etc.), ou plaque chauffante.
1

---------------------- Page: 3 ----------------------
IS0 659 : 1988 (FI
6.9 Étuve à chauffage électrique, munie d’un dispositif de 8.3 Graines de grosseur moyenne (tournesol,
thermorégulation, réglable en particulier à 103 OC f 2 OC, per-
arachide, soja, etc.)
mettant de réaliser une insufflation d‘air ou une pression
réduite. Sauf dans le cas des graines de coton à linters adhérents,
broyer l’échantillon pour analyse dans le broyeur mécanique
(6.2) préalablement bien nettoyé, jusqu’à l’obtention de parti-
6.10 Dessiccateur, contenant un agent déshydratant effi-
cules ayant au plus 2 mm dans leur plus grande dimension.
cace.
Rejeter la tête de la mouture (environ un vingtième de I’échan-
tillon), recueillir le reste, le mélanger avec soin et effectuer la
6.11 Dans le cas des graines de coton à linters adhérents, détermination sans délai.
prévoir également :
8.4 Graines de coton à linters adhérents
6.11.1 Étuve à chauffage électrique, réglable à
130 OC f. 2 OC.
Peser, à 1 mg près, dans le vase métallique (6.1 1.3) taré, envi-
ron 60 g de l’échantillon pour analyse tel quel. Placer le vase
avec les graines dans l‘étuve (6.1 1.1) préalablement chauffée à
6.11.2 Étuve à fumigation, à contrôle thermostatique pou-
vant chauffer un échantillon à 115 OC en 30 min. 130 OC et laisser sécher durant 2 h à 130 It 2 OC; ensuite, reti-
rer le vase de l’étuve et le laisser refroidir à l’air durant environ
30 min. Transférer les graines ainsi desséchées dans le vase
6.11.3 Vase métallique, à fond plat, d‘environ 100 mm de
poreux en céramique (6.1 1.4) dont la paroi intérieure et le fond
diamètre et d‘environ 40 mm de hauteur.
ont été préalablement humectés avec 1,5 ml de l’acide chlorhy-
drique (5.21, à l’aide de la pipette (6.1 1.6), en veillant à ce que
6.11.4 Vase poreux, en céramique, de forme cylindrique, de
l’acide soit complètement absorbé sans former de gouttes
68 mm de diamètre intérieur, de 80 mm de diamètre extérieur,
adhérentes. Recouvrir le vase avec le verre de montre (6.11.5)
de 85 mm de hauteur, et de 6 mm d’épaisseur de paroi et du
et le placer dans l’étuve à fumigation (6.11.2). Chauffer de
fond.
manière à atteindre en 30 min la température de 115 OC, qui ne
doit pas être dépassée, et maintenir cette température durant
encore 30 min.
6.11.5 Verre de montre, de 80 à 90 mm de diamètre.
Retirer le vase de l’étuve, laisser refroidir durant 1 h à l’air,

6.11.6 Pipette, de 2 ml, graduée en divisions de 0,l ml
peser de nouveau, à 1 mg près, les graines ainsi traitées, puis
broyer les graines dans le broyeur mécanique (6.2) et poursui-
vre comme cela est spécifié en 8.3.
7 Échantillonnage
8.5 Petites graines (lin, colza, etc.)
L’échantillonnage doit être effectué conformément à I‘ISO 542.
Mélanger avec soin l’échantillon pour analyse sans procéder à
un broyage mécanique préalable.
8 Préparation de l‘échantillon pour essai
8.1 Réduction des échantillons
9 Mode opératoire
Opérer sur l’échantillon pour analyse, obtenu selon les prescrip-
9.1 Prise d’essai
tions de I‘ISO 664. Si, avant la réduction de l‘échantillon pour
laboratoire, on a séparé les gros corps étrangers non oléagi-
neux, il en sera tenu compte dans les calculs (voir 10.1.4).
9.1.1 La prise d’essai doit être représentative de l’échantillon
Selon les stipulations du contrat, opérer sur l’échantillon pour
pour analyse.
analyse tel quel ou après séparation des impuretés.
9.1.2 Peser, à 1 mg près, environ 10 g de l‘échantillon pour
8.2 Coprah
essai (voir 8.2, 8.3, 8.4 ou 8.5, selon le cas).
Râper le produit manuellement ou, de préférence, au moyen de
NOTE - Dans le cas de l’arachide, la prise d‘essai peut être constituée
la râpe mécanique (6.3) permettant de traiter la totalité de
par des fractions séparées de graines pures, d’impuretés non oléagi-
l‘échantillon pour analyse. Si l‘on opère par râpage manuel
neuses et oléagineuses et de poussières totales, en quantités propor-
(procédé qui ne permet pas de râper tout l‘échantillon pour
tionnelles à la teneur en ces différents éléments de l‘échantillon pour
analyse), s’efforcer d‘obtenir un sous-échantillon aussi repré-
analyse tel quel
sentatif que possible et tenir compte, à cet effet, de la grosseur
et de la couleur des différents morceaux.
9.1.3 Dans le cas du coprah et des graines de grosseur
moyenne, y compris les graines de coton à linters adhérents,
La longueur des particules de la râpure doit être voisine de
transférer la prise d’essai dans la cartouche (6.5) et boucher
2 mm mais ne doit pas être supérieure à 5 mm. Mélanger la
râpure avec soin et effectuer la détermination sans délai. celle-ci avec un tampon d’ouate (6.5).
2

---------------------- Page: 4 ----------------------
IS0 659 : 19ûû (FI
chauffant les ballons durant environ 20 min dans l'étuve (6.9)
9.1.4 Dans le cas des petites graines, broyer la prise d'essai
réglée à 103 OC f 2 OC.') Faciliter l'élimination soit en insuf-
dans le microbroyeur (6.4) ou le broyeur (6.21, en ayant soin de
flant dans les ballons, par moments, de l'air ou, de préférence,
ne pas laisser de graines intactes. Transférer, sans perte, dans
un gaz inerte (tel que l'azote ou le dioxyde de carbone), soit en
la cartouche (6.51, les graines broyées, en se servant d
...

SLOVENSKI SIST ISO 659
prva izdaja
STANDARD
junij 1995
Oljnice - Dolo~anje heksanskega (ali petroleterskega) ekstrakta,
imenovanega "vsebnost olja" (prevzet standard ISO 659:1988 z
metodo platnice)
Oilseeds - Determination of hexane extract (or light petroleum extract),
called “oil content”
Graines oléagineuses - Détermination de l’extrait à l’hexane (ou à l’éther
de pétrole), dit «teneur en huile»
Deskriptorji: kmetijski pridelki, oljnice, kemi~ne analize, dolo~anje vsebnosti, heksan,
olja, ekstrakcijske metode
Referen~na {tevilka
ICS 67.200.20 SIST ISO 659:1995 (en)
Nadaljevanje na straneh od II do III in 1 do 5
© Standard je zalo`il in izdal Urad Republike Slovenije za standardizacijo in meroslovje pri Ministrstvu za znanost in tehnologijo.
Razmno`evanje ali kopiranje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST ISO 659 : 1995
UVOD
Standard SIST ISO 659, Oljnice - Dolo~anje heksanskega (ali petroleterskega) ekstrakta,
imenovanega "vsebnost olja", prva izdaja, 1995, ima status slovenskega standarda in je z
metodo platnice prevzet mednarodni standard ISO 659, Oilseeds - Determination of hexane
extract (or light petroleum extract), called “oil content”, second edition, 1988-02-15.
PREDGOVOR
Mednarodni standard ISO 659:1988 je pripravil tehni~ni odbor Mednarodne organizacije za
standardizacijo ISO/TC 34 Kmetijski pridelki in `ivilski proizvodi.
Odlo~itev za prevzem tega standarda po metodi platnice je sprejela delovna skupina WG 2 -
Oljnice ter rastlinske in `ivalske ma{~obe in olja v okviru tehni~nega odbora USM/TC
Kmetijski pridelki in `ivilski proizvodi.
Ta slovenski standard je dne 1995-06-16 odobril direktor USM.
ZVEZA S STANDARDI
Ta standard skupaj z naslednjimi slovenskimi standardi, prevzetimi mednarodnimi standardi
ISO, ureja kontrolo kakovosti oljnic ter rastlinskih in `ivalskih ma{~ob in olj:
SIST ISO 542 (en) Oljnice - Vzor~enje
SIST ISO 658 (en) Oljnice - Dolo~anje vsebnosti ne~isto~
SIST ISO 661 (en) Olja in masti rastlinskega in `ivalskega porekla - Priprava
preskusnega vzorca
SIST ISO 664 (en) Oljnice - Zmanj{anje laboratorijskega vzorca na preskusni
vzorec
SIST ISO 665 (en) Oljnice - Dolo~anje vsebnosti vlage in hlapnih snovi
SIST ISO 729 (en) Oljnice - Dolo~anje kislosti olja
SIST ISO 5508 (en) Rastlinske in `ivalske ma{~obe in olja - Dolo~anje sestave
ma{~obnih kislin z metodo plinske kromatografije
SIST ISO 5509 (en) Rastlinske in `ivalske ma{~obe in olja - Priprava metil estrov
ma{~obnih kislin
SIST ISO 5555 (en) Rastlinske in `ivalske ma{~obe in olja - Vzor~enje
OSNOVA ZA IZDAJO STANDARDA
- Prevzem standarda ISO 659:1988.
- Ta slovenski standard pokriva podro~je JUS E.B8.014:72.
OPOMBI
- Povsod, kjer se v besedilu standarda uporablja izraz "mednarodni standard", pomeni to
v SIST ISO 659:1995 "slovenski standard".
- Uvod in predgovor nista sestavni del standarda.
II

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SIST ISO 659 : 1995
Po mnenju Ministrstva za informiranje Republike Slovenije z dne 18. februarja 1992, {tev. 23/96-92, spada ta publikacija med
proizvode informativne narave iz 13. to~ke tarifne {tevilke 3, za katere se pla~uje 5-odstotni prometni davek.
III

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IS0
INTERNATIONAL STANDARD
659
Second edition
1988-02- 15
INTERNATIONAL ORGANIZATION FOR STANDARDIZATION
ORGANISATION INTERNATIONALE DE NORMALISATION
ME>KAYHAPOflHAR OPrAHM3A!&iR il0 CTAHflAPTM3AuMM
- Determination of hexane extract (or light
Oilseeds
petroleum extract), called "oil content"
Graines oléagineuses - Détermination de I'extrait à I'hexane (ou à I'éther de pétrole),
dit ((teneur en huile))
Reference number
IS0 659 : 1988 (E)

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Foreword
IS0 (the International Organization for standardization) is a worldwide federation of
national standards bodies (IS0 member bodies). The work of preparing International
Standards is normally carried out through IS0 technical committees. Each member
body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, govern-
mental and non-governmental, in liaison with EO, also take part in the work. IS0
collaborates closely with the International Electrotechnical Commission (IEC) on all
matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are circulated to
the member bodies for approval before their acceptance as International Standards by
the IS0 Council. They are approved in accordance with IS0 procedures requiring at
least 75 % approval by the member bodies voting.
International Standard IS0 659 was prepared by Technical Committee ISO/TC 34,
Agricultural food products.
This second edition cancels and replaces the first edition (IS0 659 : 19791, of which it
constitutes a minor revision.
International Organization for Standardization. 1988 0
Printed in Switzerland

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INTERNATIONAL STANDARD IS0 659 : 1988 (E)
Oilseeds - Determination of hexane extract (or light
petroleum extract), called "oil content"
5 Reagents
1 Scope
5.1 Technical n-hexane or, failing this, light petroleum,
This International Standard specifies a reference method for
essentially composed of hydrocarbons with 6 carbon atoms, of
the determination of the hexane extract (or light petroleum
which less than 5 YO distils below 50 OC and more than 95 %
extract), called "oil content", of oilseeds used as industrial raw
distils between 50 and 70 OC and which has a bromine value
materials.
less than 1. For either solvent, the residue on complete
U'
evaporation shall not exceed 2 mg per 100 ml.
NOTE - If required, the following may be analysed separately :
-
the pure seeds and the impurities (see 11.2) :
5.2 Hydrochloric acid, concentrated, e20 = 1,19 g/ml
-
in the case of groundnuts. the pure seeds, the total fines, the
(only in the case of cottonseed with adherent linters -
non-oleaginous impurities and the oleaginous impurities.
see 8.4).
2 Normative references
6 Apparatus
The following standards contain provisions which, through
Usual laboratory apparatus and in particular :
reference in this text, constitute provisions of this International
Standard. At the time of publication, the editions indicated
6.1 Analytical balance.
were valid. All standards are subject to revision, and parties to
agreements based on this International Standard are encour-
6.2 Mechanical mill, easy to clean, appropriate to the
aged to investigate the possibility of applying the most recent
nature of the oilseeds and allowing the oilseeds to be ground
editions of the standards listed below. Members of IEC and IS0
without heating or appreciable change in moisture, volatile
maintain registers of currently valid International Standards.
matter or oil content.
IS0 542 : 1980, Oilseeds - Sampling.
6.3 Mechanical grater or, failing this, hand grater (only in
the case of copra - see 8.2).
IS0 664 : 1977, Oilseeds - Reduction of contract samples to
<- analysis samples.
6.4 Mechanical micro-grinder (see 11.11, capable of pro-
ducing a fineness of grinding of oilseeds of less than 160 pm,
IS0 665 : 1977, Oilseeds - Determination of moisture and
with the exception of the "shell", particles of which may reach
volatile matter content.
400 Hm.
6.5 Extraction thimble and cotton wool, free from matter
3 Definition
soluble in hexane or light petroleum.
For the purposes of this International Standard, the following
6.6 Suitable extraction apparatus, fitted with a flask of
definition applies.
capacity 200 to 250 ml.
hexane extract, called "oil content": The whole of the
NOTE - Several flasks are necessary (see 9.3).
substances extracted under the operating conditions specified
in this International Standard, and expressed as a percentage
6.7 Pumice stone, in small particles, previously dried in an
by mass of the product as received. On request, it may be
oven at 103 OC I 2 OC and cooled in a desiccator.
expressed relative to the dry matter.
6.8 Electric heating bath (sand-bath, water-bath, etc.) or
hot-plate.
4 Principle
6.9 Electrically heated oven, with thermostatic control,
Extraction of a test portion in a suitable apparatus, with
capable of being maintained in particular at 103 OC I 2 OC,
technical hexane or, failing this, light petroleum. Elimination of
permitting ventilation or reduced pressure to be obtained.
the solvent and weighing of the extract obtained.
1

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IS0 659 : 1988 (E)
6.10 Desiccator, containing an efficient desiccant. 8.4 Cottonseed with adherent linters
Weigh, to the nearest 1 mg in the tared metal dish (6.11.31,
6.11
In the case of cottonseed with adherent linters, the
about 60 g of the analysis sample as received. Place the dish
following are also required.
and seeds in the oven (6.11.11, previously heated to 130 OC,
and leave to dry for 2 h at 130 OC f 2 OC; then remove the
6.11.1 Electrically heated oven, capable of being main-
dish from the oven and allow to cool in air for about 30 min.
tained at 130 OC f 2 OC.
Transfer the dried seeds to the porous ceramic vessel (6.11.4),
the inside walls and the base of which have been previously
moistened with 1,5 ml of the hydrochloric acid (5.2) by means
6.11.2 Fumigation oven, with a thermostatic control,
of the pipette (6.11.61, taking care that the acid is completely
capable of heating a sample to 115 OC in 30 min.
absorbed without forming adherent drops. Cover the vessel
with the watch glass (6.11.5) and place it in the fumigation
6.11.3 Metal dish, flat-bottomed, of diameter 100 mm and
oven (6.1 1.2). Heat so as to reach 115 OC in 30 min; do not heat
height approximately 40 mm.
beyond this temperature and maintain it for another 30 min.
6.11.4 Porous vessel, made of ceramic material, cylindrical, Remove the vessel from the oven, allow to cool for 1 h in air,
of internal diameter 68 mm, external diameter 80 mm, height reweigh the treated seeds to the nearest 1 mg, then grind the
85 mm, and thickness of walls and base 6 mm. seeds in the mechanical mill (6.2) and proceed as specified
in 8.3.
6.11.5 Watch glass, of diameter 80 to 90 mm.
8.5 Small seeds (linseed, colza, etc.)
6.11.6 Pipette, 2 ml, graduated in 0,l ml divisions.
Carefully mix the analysis sample without previous mechanical
grinding.
7 Sampling
Sampling shall be carried out in accordance with IS0 542.
9 Procedure
8 Preparation of test sample
9.1 Test portion
8.1 Reduction of sample
9.1.1 The test portion shall be representative of the analysis
sample.
Take an analysis sample obtained in accordance with IS0 664.
If large non-oleaginous foreign bodies have been separated
1 mg, about 10 g of the test sam-
9.1.2 Weigh, to the nearest
before the reduction of the laboratory sample, make allowance
ple (see 8.2, 8.3, 8.4 or 8.5, as appropriate).
for this in the calculation (see 10.1.4). According to-the re-
quirements of the contract, use an analysis sample as received
NOTE - In the case of groundnuts. the test portion may comprise the
or after separation of the impurities.
separated fractions of pure seeds. non-oleaginous and oleaginous im-
purities, and the total fines in quantities proportional to those of the dif-
-
8.2 Copra
ferent constituents in the analysis sampl
...

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