SIST EN ISO 7827:2013

SLOVENSKI STANDARD
SIST EN ISO 7827:2013
01-januar-2013
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SIST EN ISO 7827:1997
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Water quality - Evaluation of the "ready", "ultimate" aerobic biodegradability of organic
compounds in an aqueous medium - Method by analysis of dissolved organic carbon
(DOC) (ISO 7827:2010)
Wasserbeschaffenheit - Bestimmung der "leichten", "vollständigen" aeroben biologischen
Abbaubarkeit organischer Stoffe in einem wässrigen Medium - Verfahren mittels Analyse
des gelösten organischen Kohlenstoffs (DOC) (ISO 7827:2010)
Qualité de l'eau - Évaluation de la biodégradabilité aérobie «facile», «ultime» des
composés organiques en milieu aqueux - Méthode par analyse du carbone organique
dissous (COD) (ISO 7827:2010)
Ta slovenski standard je istoveten z: EN ISO 7827:2012
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN ISO 7827:2013 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 7827:2013

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SIST EN ISO 7827:2013


EUROPEAN STANDARD
EN ISO 7827

NORME EUROPÉENNE

EUROPÄISCHE NORM
December 2012
ICS 13.060.70 Supersedes EN ISO 7827:1995
English Version
Water quality - Evaluation of the "ready", "ultimate" aerobic
biodegradability of organic compounds in an aqueous medium -
Method by analysis of dissolved organic carbon (DOC) (ISO
7827:2010)
Qualité de l'eau - Évaluation de la biodégradabilité aérobie Wasserbeschaffenheit - Bestimmung der leichten,
"facile", "ultime" des composés organiques en milieu vollständigen aeroben biologischen Abbaubarkeit
aqueux - Méthode par analyse du carbone organique organischer Stoffe in einem wässrigen Medium - Verfahren
dissous (COD) (ISO 7827:2010) mittels Analyse des gelösten organischen Kohlenstoffs
(DOC) (ISO 7827:2010)
This European Standard was approved by CEN on 8 December 2012.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2012 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 7827:2012: E
worldwide for CEN national Members.

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SIST EN ISO 7827:2013
EN ISO 7827:2012 (E)
Contents Page
Foreword . 3

2

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SIST EN ISO 7827:2013
EN ISO 7827:2012 (E)
Foreword
The text of ISO 7827:2010 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 7827:2012 by
Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by June 2013, and conflicting national standards shall be withdrawn at
the latest by June 2013.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 7827:1995.
According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 7827:2010 has been approved by CEN as a EN ISO 7827:2012 without any modification.


3

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SIST EN ISO 7827:2013

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SIST EN ISO 7827:2013

INTERNATIONAL ISO
STANDARD 7827
Third edition
2010-11-15

Water quality — Evaluation of the
“ready”, “ultimate” aerobic
biodegradability of organic compounds
in an aqueous medium — Method by
analysis of dissolved organic carbon
(DOC)
Qualité de l'eau — Évaluation de la biodégradabilité aérobie «facile»,
«ultime» des composés organiques en milieu aqueux — Méthode par
analyse du carbone organique dissous (COD)



Reference number
ISO 7827:2010(E)
©
ISO 2010

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SIST EN ISO 7827:2013
ISO 7827:2010(E)
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ii © ISO 2010 – All rights reserved

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SIST EN ISO 7827:2013
ISO 7827:2010(E)
Contents Page
Foreword .iv
1 Scope.1
2 Normative references.1
3 Terms and definitions .2
4 Principle .3
5 Test environment.3
6 Reagents .3
7 Apparatus.4
8 Procedure.5
9 Calculation and expression of results .7
10 Validity of the test.8
11 Test report.9
Annex A (informative) Typical degradation curve .10
Annex B (informative) Interpretation of results .11
Bibliography.13

© ISO 2010 – All rights reserved iii

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SIST EN ISO 7827:2013
ISO 7827:2010(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 7827 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological
methods.
This third edition cancels and replaces the second edition (ISO 7827:1994), which has been technically
revised.
iv © ISO 2010 – All rights reserved

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SIST EN ISO 7827:2013
INTERNATIONAL STANDARD ISO 7827:2010(E)

Water quality — Evaluation of the “ready”, “ultimate” aerobic
biodegradability of organic compounds in an aqueous
medium — Method by analysis of dissolved organic carbon

(DOC)
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This standard does not purport to address all of the safety problems, if any, associated with
its use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this International Standard
be carried out by suitably trained staff.
SAFETY PRECAUTIONS — Activated sludge and sewage contain potentially pathogenic organisms.
Therefore take appropriate precautions when handling them. Handle toxic test compounds and those
whose properties are unknown with care.
1 Scope
This International Standard specifies a method for the evaluation of the “ready” and “ultimate” biodegradability
of organic compounds at a given range of concentrations by aerobic microorganisms. In this context, this
International Standard also gives specific definitions for the terms “ready” and “ultimate”.
The method applies to organic compounds which are:
a) soluble at the concentration used under the conditions of the test [dissolved organic carbon (DOC)
concentrations of 10 mg/l to 40 mg/l];
b) non-volatile or having a negligible vapour pressure under the conditions of the test;
c) not significantly adsorbable on glass and activated sludge;
d) not inhibitory to the test microorganisms at the concentration chosen for the test.
The method is not suitable for waste waters, as they usually contain significant amounts of water-insoluble
organic carbon, which is not included in DOC measurements.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 8245, Water quality — Guidelines for the determination of total organic carbon (TOC) and dissolved
organic carbon (DOC)
ISO 9408, Water quality — Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous
medium by determination of oxygen demand in a closed respirometer
© ISO 2010 – All rights reserved 1

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SIST EN ISO 7827:2013
ISO 7827:2010(E)
ISO 9439, Water quality — Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous
medium — Carbon dioxide evolution test
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
degradation time
t
2
time from the end of the lag time, t , until the time that about 90 % of the maximum level of biodegradation has
1
been reached
NOTE Degradation time is expressed in days.
3.2
inherent biodegradation
level of biodegradation achieved which indicates the test compound is unlikely to be persistent in the
environment
NOTE See Annex B.
3.3
lag time
t
1
time from the start of the test until 10 % biodegradation has been reached
NOTE Lag time is expressed in days.
3.4
maximum level of biodegradation
degree of biodegradation of a chemical compound or organic matter in a test above which no further
biodegradation takes place during the test
3.5
primary biodegradation
structural change (transformation) of a chemical compound by microorganisms resulting in the loss of a
specific property of that compound
3.6
“ready” biodegradation
level of biodegradation achieved under defined conditions which indicates the test compound is considered
likely to degrade rapidly and completely under aerobic aquatic environmental conditions
NOTE See Annex B.
3.7
suspended solids
〈activated sludge〉 solid material within activated sludge with a particle diameter of >45 µm
NOTE The concentration of suspended solids is obtained by filtration or centrifugation of a known volume of sludge
under specified conditions, drying at 105 °C, and correcting for the volume of sample. The concentration of suspended
solids is expressed in milligrams per litre.
3.8
“ultimate” biodegradation
breakdown of a chemical compound or organic matter by microorganisms to carbon dioxide, water and
mineral salts of any other elements present (mineralization), and the production of new biomass
2 © ISO 2010 – All rights reserved

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SIST EN ISO 7827:2013
ISO 7827:2010(E)
4 Principle
The biodegradation of organic compounds by aerobic microorganisms in a mineral medium is determined by
measurement of the DOC concentration. The organic compound is the sole source of carbon in the medium.
The concentration of the compound used is such that the initial DOC in the medium is between 10 mg/l and
40 mg/l. If necessary, concentrations greater than 40 mg/l may be used. The test solution is aerated in the
dark or diffuse light at 22 °C ± 2 °C.
Biodegradation is monitored by measurement of the DOC at the start (day 0), at the end of the test (day 28 or
longer if necessary), and at a minimum of three intermediate time intervals. The percentage removal of DOC
is calculated at each time interval, and the biodegradability of the organic compound based on these data.
Specific analysis can give additional information on primary biodegradation.
The test is not suitable for compounds which are inhibitory at the concentration used in the test. Inhibitory
effects can be determined as specified in 8.3 or by using any other method for determining the inhibitory effect
[1]
on bacteria of a substance (e.g. ISO 8192 ).
The conditions specified in this International Standard do not necessarily correspond to the optimal conditions
allowing the maximum degree of biodegradation to occur. Tests for ready biodegradability have very stringent
conditions and a substance which passes these is considered likely to be rapidly and completely degraded in
any aerobic aquatic environmental compartment, especially in waste water treatment plants. For alternative
[6]
biodegradation methods, see ISO/TR 15462 .
See Annex B for information on interpretation of results.
5 Test environment
Incubation shall take place in the dark or in diffused light in an enclosure which is maintained at 22 °C ± 2 °C
and which is free from vapours that are toxic to microorganisms.
6 Reagents
Use only reagents of recognized analytical grade (where applicable).
6.1 Water, distilled or demineralized, containing less than 10 % of the initial DOC content introduced by the
compound to be tested to maintain acceptable precision.
6.2 Test medium
6.2.1 Composition
6.2.1.1 Solution A
Anhydrous potassium dihydrogenphosphate, KH PO 8,5 g
2 4
Anhydrous dipotassium hydrogenphosphate, K HPO 21,75 g
2 4
Disodium hydrogenphosphate dihydrate, Na HPO ·2HO 33,4 g
2 4 2
Ammonium chloride, NHCl 0,5 g
4
Water (6.1), quantity necessary to make up to 1 000 ml
Measure the pH value of the solution, which should be 7,4 ± 0,2. If it is not, then prepare a new solution.
© ISO 2010 – All rights reserved 3

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SIST EN ISO 7827:2013
ISO 7827:2010(E)
6.2.1.2 Solution B
Dissolve 22,5 g of magnesium sulfate heptahydrate (MgSO ·7H O) in water (6.1) and make up to 1 000 ml.
4 2
6.2.1.3 Solution C
Dissolve 27,5 g of anhydrous calcium chloride (CaCl ) in water (6.1) and make up to 1 000 ml.
2
6.2.1.4 Solution D
Dissolve 0,25 g of iron(III) chloride hexahydrate (FeCl ·6H O) in water (6.1) and make up to 1 000 ml. Prepare
3 2
the solution freshly just before use.
The need to prepare this solution just before use can be avoided if a drop of concentrated hydrochloric acid
(HCI) or 0,4 g/l of ethylenediaminetetraacetic acid (EDTA) is added.
6.2.2 Preparation
For 1 l of test medium, add to about 500 ml of water (6.1):
a) 1 ml of each of solutions B, C, and D;
b) 10 ml of solution A.
Make up to 1 000 ml with water (6.1). Solution A is added last, to avoid precipitation of salts. Prepare the test
medium freshly before use. Solutions A to C can be stored for up to 6 months in the dark at
...