SIST EN 16343:2013

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.NRKosmetische Mittel - Untersuchung von kosmetischen Mitteln - Bestimmung von 3-Iod-2-propinylbutylcarbamat (IPBC) in kosmetischen Mitteln, LC-MS-VerfahrenCosmétiques - Analyse de produits cosmétiques - Détermination quantitative du carbamate 3-iodo-2-propynylbutyle (IPBC), méthodes CL-SMCosmetics - Analysis of cosmetic products - Determination of 3-iodo-2-propynyl butylcarbamate (IPBC) in cosmetic preparations, LC-MS methods71.100.70SULSRPRþNLCosmetics. ToiletriesICS:Ta slovenski standard je istoveten z:EN 16343:2013SIST EN 16343:2013en,fr,de01-julij-2013SIST EN 16343:2013SLOVENSKI
STANDARD



SIST EN 16343:2013



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16343
May 2013 ICS 71.100.70 English Version
Cosmetics - Analysis of cosmetic products - Determination of 3-iodo-2-propynyl butylcarbamate (IPBC) in cosmetic preparations, LC-MS methods
Cosmétiques - Analyse des produits cosmétiques - Détermination quantitative du carbamate 3-iodo-2-propynylbutyle (IPBC), méthodes CL-SM
Kosmetische Mittel - Untersuchung von kosmetischen Mitteln - Bestimmung von 3-Iod-2-propinylbutylcarbamat (IPBC) in kosmetischen Mitteln, LC-MS-Verfahren This European Standard was approved by CEN on 25 April 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
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B-1000 Brussels © 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16343:2013: ESIST EN 16343:2013



EN 16343:2013 (E) 2 Contents Page Foreword . 3 1 Scope . 4 2 Principle. 4 3 Reagents . 4 4 Apparatus and equipment . 5 5 Procedure . 5 6 Evaluation . 7 7 Test report . 7 Annex A (informative) Results of the inter-laboratory test . 8 Bibliography. 9
SIST EN 16343:2013



EN 16343:2013 (E) 3 Foreword This document (EN 16343:2013) has been prepared by Technical Committee CEN/TC 392 “Cosmetics”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 2013, and conflicting national standards shall be withdrawn at the latest by November 2013. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. SIST EN 16343:2013



EN 16343:2013 (E) 4 1 Scope This European Standard specifies a method for the quantitative determination of 3-iodo-2-propynyl butylcarbamate (IPBC) in the concentration range from 0,005 g/100 g to 0,1 g/100 g - Annex V No. 56 in Regulation (EC) No 1223/2009 on cosmetic products. 2 Principle IPBC is extracted from the cosmetic preparation using methanol. IPBC present in the sample extract is separated using reverse phase HPLC with mass specific detection (LC-MS or LC-MS/MS). Quantitative determination of IPBC is made using the external standard method of calibration or standard addition. 3 Reagents 3.1 General If not otherwise specified, analytical-grade chemicals shall be used; water shall be distilled or of a suitable purity. “Solution” shall be understood as an aqueous solution unless otherwise specified. 3.2 Iodopropynyl butylcarbamat, CAS number: 55406-53-6 (Supplier : Sigma-Aldrich1) (521949), Dr. Ehrenstorfer GmbH1) (C 14335000)). 3.3 Methanol, HPLC grade, CAS number: 67-56-1. 3.4 Formic acid, CAS number: 64-18-6. 3.5 Tetrahydrofuran (THF), CAS number: 109-99-9. 3.6 Propan-2-ol, CAS number: 67-63-0. 3.7 Eluents 3.7.1 Eluent A, 1 ml of formic acid (3.4) is mixed with 1 000 ml of water. 3.7.2 Eluent B, Methanol (3.3). 3.8 IPBC stock solution, ρ = 1 mg/ml. Weigh approximately 0,05 g of IPBC (3.2) into a 50-ml-volumetric flask. Firstly, dissolve in a small amount of methanol (3.3) and then fill up to the calibration mark with methanol. This solution has a shelf life of 8 weeks if it is stored in a refrigerator. 3.9 Calibration solutions (standard solutions) 5,0 ml of the stock solution (3.8) is transferred into a 50-ml-volumetric flask and fill
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